Regulatory

Part:BBa_K553021:Design

Designed by: Luca Braga, Niels Ntamati   Group: iGEM11_UNITS_Trieste   (2011-09-17)

Mammalian Tra-R inducible element


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When combined to have different repeats (1 to 7) and associated to a minimal CMV promoter (CMVmin, BBa_553022), it can be used as a mammalian TraR-inducible gene expression system

Source

DNA Synthesis

References

1) Neddermann P, Gargioli C, Muraglia E, Sambucini S, Bonelli F, De Francesco R, Cortese R. A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR. EMBO Rep. 2003 Feb;4(2):159-65.

2) Deepak Chandran, Frank T Bergmann and Herbert M Sauro. "TinkerCell: modular CAD tool for synthetic biology". Journal of Biological Engineering 2009, 3:19 DOI: 10.1186/1754-1611-3-1